深夜福利在线观看视频,激情五月综合开心五月,亚洲大尺码专区在线观看,日本一区二区三区麻烦视频,永久天堂网av手机版不卡,在线欧美日韩国产精品,国产中文字幕在线观看免费,日韩精品视频在线观看一区二区三区,美妇av一区二区三区

fermentas逆轉錄試劑盒RevertAid First Strand cDNA Synthesis Kit說明書

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit是一套用于以RNA為模板高效合成*鏈cDNA的完整系統，可合成長達13kb的cDNA。試劑盒配套提供的RiboLockRNase Inhibitor，可有效防止RNA模板的降解。試劑盒配套提供oligo(dT)18引物和隨機六聚體引物。oligo(dT)18引物可以選擇性地與mRNA中的poly (A)尾結合。隨機六聚體引物無需poly (A)尾，因此可轉錄mRNA 5’-端區域，也可以無poly (A)尾的RNA (如micoRNA)為模板合成cDNA。該試劑盒也可使用基因特異性引物。優點：

合成全長的*鏈cDNA，zui長可合成13kb的cDNA*反應溫度為42℃*一提供RT反應所需的所有組分

應用:

*鏈cDNA合成，作為RT-PCR和實時RT-qPCR的模板構建全長cDNA文庫反義RNA合成

fermentas逆轉錄試劑盒RevertAid First Strand cDNA Synthesis Kit

品名	貨號	品牌	規格	售價
RevertAid?First Strand cDNA Synthesis Kit	K1622	Fermentas（MBI）	100 react	詢價

RevertAid First Strand cDNA Synthesis KitFeatures·Full-lengthfirst strand cDNA up to 13 kb.·Increased reaction temperaturesin the range of 42-50°C.·Supplied withthe recombinant RiboLock?RNase Inhibitor.·Complete–oligo(dT)18and random hexamer primers included with the kit.Applications·First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2).·Construction of full length cDNA libraries.·Generation of probes for hybridization.·aRNA synthesis.DescriptionThe RevertAid?First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb.The kit uses RevertAid?Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase.The recombinant RiboLock?RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C.The kit is supplied with both oligo(dT)18and random hexamer primers. The oligo(dT)18anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail e.g. micro RNAs. Gene-specific primers may also be used with the kits.The first strand of cDNA can be directly used as a template in PCR (seeRT-PCR protocol), real-time PCR or in second strand cDNA synthesis (seeprotocol).Quality ControlThe kit is functionally tested in RT-PCR using 100 fg control GAPDH RNA and GAPDH control primers generated a 496 bp product visible on agarose gel after ethidium bromide staining.

is Kit

RevertAid First Strand cDNA Synthesis Kit合成全長cDNA1 μg小鼠心臟總RNA作為模板，用oligo(dT)18 引物進行逆轉錄反應。由此合成的cDNA，再作為Taq DNA Polymerase后續PCR擴增的模板。末端2024 bp片段的成功擴增，表明長度為13.8 kb的全長RNA片段得到成功的逆轉錄。產品組成RevertAid First Strand cDNA Synthesis Kit包含RevertAid Reverse Transcriptase、RiboLock RNase Inhibitor、5x Reaction Buffer、dNTP Mix、Oligo(dT)18 引物、隨機六聚體引物、對照GAPDH RNA、10 μM 正向GAPDH引物、10 μM反向GAPDH 引物以及nuclease-free water。